Detection of Cryptosporidium parvum DNA in human feces by nested PCR
نویسندگان
چکیده
منابع مشابه
Species-specific, nested PCR-restriction fragment length polymorphism detection of single Cryptosporidium parvum oocysts.
Concurrent with recent advances seen with Cryptosporidium parvum detection in both treated and untreated water is the need to properly evaluate these advances. A micromanipulation method by which known numbers of C. parvum oocysts, even a single oocyst, can be delivered to a test matrix for detection sensitivity is presented. Using newly developed nested PCR-restriction fragment length polymorp...
متن کاملPCR and real time PCR for the detection of Cryptosporidium parvum oocyst DNA.
Three DNA extraction kits were used, all without preliminary procedures, then DNA extraction was preceded with freeze/thaw cycles in three versions. A lack of desired effect resulted in the application of liquid nitrogen/water bath cycles before the use of the extractions in further experiments. The effectiveness of DNA extraction was measured by PCR signal and C(T) values of real time PCR. A c...
متن کاملa nested-pcr assay for detection of cryptosporidium parvum oocysts in water samples
cryptosporidiosis is a gastroenteric disease caused by the protozoan parasite cryptosporidium parvum. water–borne transmission of this organism has become more prevalent in recent years. current method for detection of c. parvum oocysts in water is immunofluoresence assay (ifa). the method is time consuming, laborious and particularly not–specific. it cannot determine the infectivity of detecte...
متن کاملDetection of Cryptosporidium parvum oocysts in bovine feces by monoclonal antibody capture enzyme-linked immunosorbent assay.
A monoclonal antibody enzyme-linked immunosorbent assay (ELISA) was developed to detect Cryptosporidium parvum oocysts in bovine feces. Fecal oocysts were concentrated by centrifugation through Formalin-ethyl acetate solution and captured with monoclonal antibody 18.280.2 reactive with C. parvum oocysts. Captured oocysts were detected with goat anti-oocyst serum, following the addition of a per...
متن کاملComparison of PCR and microscopy for detection of Cryptosporidium parvum in human fecal specimens: clinical trial.
PCR technology offers alternatives to conventional diagnosis of Cryptosporidium for both clinical and environmental samples. We compared microscopic examination by a conventional acid-fast staining procedure with a recently developed PCR test that can not only detect Cryptosporidium but is also able to differentiate between what appear to be host-adapted genotypes of the parasite. Examinations ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1996
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.34.7.1769-1772.1996